Isolation of mitochondria from tissue culture cells.
نویسندگان
چکیده
The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a tight-fitting pestle, and mitochondria are isolated by differential centrifugation.
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ورودعنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2014 10 شماره
صفحات -
تاریخ انتشار 2014